Sorry, I don't understand your search. ×
Back to Search Start Over

Biofilm-based biocatalysis for β-cyclodextrin production by the surface-display of β-cyclodextrin glycosyltransferase in Bacillus subtilis

Authors :
Dan Wang
Sinan Wang
Wenjun Sun
Tianpeng Chen
Caice Liang
Pengpeng Yang
Qingguo Liu
Chunguang Zhao
Yong Chen
Source :
Scientific Reports, Vol 14, Iss 1, Pp 1-11 (2024)
Publication Year :
2024
Publisher :
Nature Portfolio, 2024.

Abstract

Abstract β-cyclodextrin (β-CD) is an important cyclic oligosaccharide, which is widely applicated in foods, environmental protection, and cosmetics, primarily prepared from enzymatic synthesis in traditional industry. However, several challenges persist, including cumbersome processes and difficulties in achieving continuous fermentation and catalysis. This research introduced a biofilm-based immobilized fermentation, integrating with enzyme catalysis system of surface display in Bacillus subtilis. The bslA gene was selected to construct the surface display system due to its ability to promote biofilm formation and serve as an anchorin. Compared to free cell catalysis, the biofilm-based immobilized catalysis expanded the temperature range to 40–70 and the pH range to 5–7.5. During the continuous catalysis process, by the 13th batch, the relative activity remained around 52%, and the conversion rate exceeded 36%, similar to the single-batch free cell catalysis. These findings provide valuable insights and effective strategies for the industrial production of β-CD and other biochemicals through continuous catalysis.

Details

Language :
English
ISSN :
20452322
Volume :
14
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Scientific Reports
Publication Type :
Academic Journal
Accession number :
edsdoj.6a7367a3f78647a2a83a39a9e2054e6c
Document Type :
article
Full Text :
https://doi.org/10.1038/s41598-024-81490-z