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The time revolution in macromolecular crystallography

Authors :
Georgii Khusainov
Joerg Standfuss
Tobias Weinert
Source :
Structural Dynamics, Vol 11, Iss 2, Pp 020901-020901-17 (2024)
Publication Year :
2024
Publisher :
AIP Publishing LLC and ACA, 2024.

Abstract

Macromolecular crystallography has historically provided the atomic structures of proteins fundamental to cellular functions. However, the advent of cryo-electron microscopy for structure determination of large and increasingly smaller and flexible proteins signaled a paradigm shift in structural biology. The extensive structural and sequence data from crystallography and advanced sequencing techniques have been pivotal for training computational models for accurate structure prediction, unveiling the general fold of most proteins. Here, we present a perspective on the rise of time-resolved crystallography as the new frontier of macromolecular structure determination. We trace the evolution from the pioneering time-resolved crystallography methods to modern serial crystallography, highlighting the synergy between rapid detection technologies and state-of-the-art x-ray sources. These innovations are redefining our exploration of protein dynamics, with high-resolution crystallography uniquely positioned to elucidate rapid dynamic processes at ambient temperatures, thus deepening our understanding of protein functionality. We propose that the integration of dynamic structural data with machine learning advancements will unlock predictive capabilities for protein kinetics, revolutionizing dynamics like macromolecular crystallography revolutionized structural biology.

Subjects

Subjects :
Crystallography
QD901-999

Details

Language :
English
ISSN :
23297778
Volume :
11
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Structural Dynamics
Publication Type :
Academic Journal
Accession number :
edsdoj.6b048fda9254b4bb19ba83f4a1908ae
Document Type :
article
Full Text :
https://doi.org/10.1063/4.0000247