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Fibroblasts alter the physical properties of dermal ECM-derived hydrogels to create a pro-angiogenic microenvironment

Authors :
Meng Zhang
Fenghua Zhao
Xue Zhang
Linda A. Brouwer
Janette K. Burgess
Martin C. Harmsen
Source :
Materials Today Bio, Vol 23, Iss , Pp 100842- (2023)
Publication Year :
2023
Publisher :
Elsevier, 2023.

Abstract

This study aimed to investigate the impact of fibroblasts (MRC-5) on the extracellular matrix (ECM) microenvironment of endothelial cells (ECs) during the vascularization of skin-derived ECM hydrogel in vitro. Two types of ECs were studied: human dermal microvascular endothelial cells (HMEC) and human pulmonary microvascular endothelial cells (HPMEC). Results showed that the presence of MRC-5 fibroblasts increased the stiffness of the hydrogel and led to larger fiber diameters and increased porosity. Extensive collagen fiber remodeling occurred in the ECM hydrogel with MRC-5 fibroblasts. Additionally, higher levels of fibulin-1 and fibronectin were deposited in the hydrogel when co-cultured with MRC-5 fibroblasts. These findings suggest that MRC-5 fibroblasts play a role in modifying the ECM microenvironment, promoting vascularization through dynamic ECM remodeling.

Details

Language :
English
ISSN :
25900064
Volume :
23
Issue :
100842-
Database :
Directory of Open Access Journals
Journal :
Materials Today Bio
Publication Type :
Academic Journal
Accession number :
edsdoj.6b3deb400e7d474382bb547b99acc898
Document Type :
article
Full Text :
https://doi.org/10.1016/j.mtbio.2023.100842