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Comparative Proteomics Analysis of Phosphine-Resistant and Phosphine-Susceptible Sitophilus oryzae (Coleoptera: Curculionidae)

Authors :
Hyun-Na Koo
Seung Ju Seok
Hyun Kyung Kim
Gil-Hah Kim
Jeong Oh Yang
Source :
Applied Sciences, Vol 11, Iss 9, p 4163 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

A proteomic method combining two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF) was used to compare the protein expression profiles of phosphine-resistant (PH3-R) and -susceptible (PH3-S) strains of Sitophilus oryzae. Thirty-nine differentially expressed protein spots were identified between the PH3-R and PH3-S strains; 20 protein spots were upregulated, and 19 protein spots were downregulated in the PH3-R strain compared with their expression in the PH3-S strain. In particular, cytochrome oxidase subunit I showed 15-fold higher expression in the PH3-R strain than in the PH3-S strain. Additionally, citrate synthase 2, delta-1-pyrolline-5-carboxylate dehydrogenase, and triose-phosphate isomerase were highly expressed in the PH3-R strain. In summary, our study has improved understanding of the molecular mechanisms of phosphine resistance in the rice weevil.

Details

Language :
English
ISSN :
20763417 and 45141487
Volume :
11
Issue :
9
Database :
Directory of Open Access Journals
Journal :
Applied Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.6bc6e4c45141487f9f4c1eee86f3a57e
Document Type :
article
Full Text :
https://doi.org/10.3390/app11094163