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DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability

Authors :
Kevin Kramm
Tim Schröder
Jerome Gouge
Andrés Manuel Vera
Kapil Gupta
Florian B. Heiss
Tim Liedl
Christoph Engel
Imre Berger
Alessandro Vannini
Philip Tinnefeld
Dina Grohmann
Source :
Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
Publication Year :
2020
Publisher :
Nature Portfolio, 2020.

Abstract

TATA-binding protein (TBP) and a transcription factor (TF) IIB-like factor are important constituents of all eukaryotic initiation complexes. Here, the authors use a DNA origami-based force clamp to investigate the assembly dynamics of human initiation complexes in the RNAP II and RNAP III systems at the single-molecule level under pico newton forces.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
11
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.6c52a4bb73744eafa2b7a2b2daca7321
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-020-16702-x