Back to Search Start Over

Optimized CRISPR-RfxCas13d system for RNA targeting in zebrafish embryos

Authors :
Luis Hernandez-Huertas
Gopal Kushawah
Alejandro Diaz-Moscoso
Laura Tomas-Gallardo
Ismael Moreno-Sanchez
Gabriel da Silva Pescador
Ariel A. Bazzini
Miguel A. Moreno-Mateos
Source :
STAR Protocols, Vol 3, Iss 1, Pp 101058- (2022)
Publication Year :
2022
Publisher :
Elsevier, 2022.

Abstract

Summary: CRISPR-Cas systems have been used to induce DNA mutagenesis for gene function discovery. However, the development of tools to eliminate RNAs provides complementary and unique approaches to disrupt gene expression. Here, we present a workflow to perform specific, efficient, and cost-effective mRNA knockdown in zebrafish embryos using our in vivo optimized CRISPR-RfxCas13d (CasRx) system. Although the described protocol focuses on mRNA knockdown in zebrafish embryos, it can also be applied to other vertebrates.For complete details on the use and execution of this protocol, please refer to Kushawah et al. (2020).

Details

Language :
English
ISSN :
26661667
Volume :
3
Issue :
1
Database :
Directory of Open Access Journals
Journal :
STAR Protocols
Publication Type :
Academic Journal
Accession number :
edsdoj.6ccc12cb9aa841758da21124aae9cd44
Document Type :
article
Full Text :
https://doi.org/10.1016/j.xpro.2021.101058