A multi‐omics approach to overeating and inactivity‐induced muscle atrophy in db/db mice

Abstract Background Overeating and inactivity are associated with type 2 diabetes. This study aimed to investigate its pathological basis using integrated omics and db/db/mice, a model representing this condition. Methods The study involved housing 8‐week‐old db/m and db/db mice for 8 weeks. Various analyses were conducted, including gene expression in skeletal muscle and small intestine using next‐generation sequencing; cytokine arrays of serum; assessment of metabolites in skeletal muscle, stool, and serum; and analysis of the gut microbiota. Histone modifications in small intestinal epithelial cells were profiled using CUT&Tag. Results Compared with db/m mice, db/db mice had 22.4% lower grip strength and approximately five times the visceral fat weight (P