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Photoprotective activity of a cream containing lyophilized aqueous extract of Lepidium meyenii (MACA) against ultraviolet irradiation on mouse skin

Authors :
Malena Castañeda-Alarcón
Carlos Bell-Cortez
Julio Hidalgo-Ascensios
Luis Moreno-Exebio
Source :
Revista Peruana de Medicina Experimental y Salud Pública, Vol 38, Iss 3, Pp 434-41 (2021)
Publication Year :
2021
Publisher :
Instituto Nacional de Salud, 2021.

Abstract

Objectives: To evaluate the photoprotective activity of a cream with lyophilized aqueous extract of maca (LEM) against ultraviolet (UV) irradiation in the skin of mice. Materials and methods: An experimental study was carried out on 35 BALB/c mice. Treatment was applied topically on the dorsum of the animals, which were subsequently irradiated with ultraviolet B rays, and then we measured the thickness in microns (μm) of histological samples of the skin of the mice. Seven groups were assigned, divided into non-irradiated: white (G1) and irradiated with UV light: no treatment (G2); with commercial sunscreen with sun protection factor (SPF) 30 (G3); cream (placebo) (G4); LEM at 15% in water (G5); LEM cream at 5% (G6); and LEM cream at 15% (G7). In vitro SPF was determined using the Mansur method. Absorbance readings were taken in an ultravioletvisible spectrophotometer (UV-VIS) and SPFs were determined for the following formulations: LEM cream at 5%, benzophenone-4 (BZF-4) and commercial sunscreen SPF 30. Results: Mouse skin thickness in microns (μm) was 27.28 in G2; 18.31 in G3; 27.33 in G4; 19.51 in G5 and 18.04 in G6. There was no significant difference between the group not exposed to radiation (G1) and the 15% LEM cream group (G7), both had the lowest thicknesses (12.76 and 14.20 μm, respectively). The SPF of LEM cream at 15% was 5.480 ± 0.020. Conclusions: The formulation with LEM cream showed photoprotective activity against UV irradiation, alkaloids were the phytochemical components mostly found and the formulation was compatible with the active principle (LEM).

Details

Language :
Spanish; Castilian
ISSN :
17264634 and 17264642
Volume :
38
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Revista Peruana de Medicina Experimental y Salud Pública
Publication Type :
Academic Journal
Accession number :
edsdoj.6d908cff33842e6829e48e9b0f30062
Document Type :
article
Full Text :
https://doi.org/10.17843/rpmesp.2021.383.7420