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Alt a 1 Promotes Allergic Asthma In Vivo Through TLR4-Alveolar Macrophages

Authors :
Guadalupe Hernandez-Ramirez
Diego Pazos-Castro
Zulema Gonzalez-Klein
Jose Luis Resuela-Gonzalez
Sergio Fernandez-Bravo
Lucia Palacio-Garcia
Vanesa Esteban
Maria Garrido-Arandia
Jaime Tome-Amat
Araceli Diaz-Perales
Source :
Frontiers in Immunology, Vol 13 (2022)
Publication Year :
2022
Publisher :
Frontiers Media S.A., 2022.

Abstract

The mold Alternaria alternata is one of the main sources of asthma exacerbation, being its major allergen, Alt a 1, indispensable for its development. The main objective of this work was to answer two main questions: 1) can Alt a 1 by itself (without any other context) induce an asthmatic profile in vivo?; and 2) Which molecular mechanisms take place during this phenomenon? To answer both questions, we have developed a mouse model of allergic asthma using only Alt a 1 for mice sensitization. We also made use of in-vitro cellular models and computational studies to support some aspects of our hypothesis. Our results showed that Alt a 1 can induce an asthmatic phenotype, promoting tissue remodeling and infiltration of CD45+ cells, especially eosinophils and macrophages (Siglec F+ and F4/80+). Also, we have found that Alt a 1 sensitization is mediated by the TLR4-macrophage axis.

Details

Language :
English
ISSN :
16643224
Volume :
13
Database :
Directory of Open Access Journals
Journal :
Frontiers in Immunology
Publication Type :
Academic Journal
Accession number :
edsdoj.6ec1b4f30ba447a3956e36249081be15
Document Type :
article
Full Text :
https://doi.org/10.3389/fimmu.2022.877383