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Assembly, Activation, and Helicase Actions of MCM2-7: Transition from Inactive MCM2-7 Double Hexamers to Active Replication Forks

Authors :
Zhiying You
Hisao Masai
Source :
Biology, Vol 13, Iss 8, p 629 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

In this review, we summarize the processes of the assembly of multi-protein replisomes at the origins of replication. Replication licensing, the loading of inactive minichromosome maintenance double hexamers (dhMCM2-7) during the G1 phase, is followed by origin firing triggered by two serine–threonine kinases, Cdc7 (DDK) and CDK, leading to the assembly and activation of Cdc45/MCM2-7/GINS (CMG) helicases at the entry into the S phase and the formation of replisomes for bidirectional DNA synthesis. Biochemical and structural analyses of the recruitment of initiation or firing factors to the dhMCM2-7 for the formation of an active helicase and those of origin melting and DNA unwinding support the steric exclusion unwinding model of the CMG helicase.

Details

Language :
English
ISSN :
20797737
Volume :
13
Issue :
8
Database :
Directory of Open Access Journals
Journal :
Biology
Publication Type :
Academic Journal
Accession number :
edsdoj.6edabcb6f52543b6800ba6884e04339e
Document Type :
article
Full Text :
https://doi.org/10.3390/biology13080629