Back to Search
Start Over
Assembly, Activation, and Helicase Actions of MCM2-7: Transition from Inactive MCM2-7 Double Hexamers to Active Replication Forks
- Source :
- Biology, Vol 13, Iss 8, p 629 (2024)
- Publication Year :
- 2024
- Publisher :
- MDPI AG, 2024.
-
Abstract
- In this review, we summarize the processes of the assembly of multi-protein replisomes at the origins of replication. Replication licensing, the loading of inactive minichromosome maintenance double hexamers (dhMCM2-7) during the G1 phase, is followed by origin firing triggered by two serine–threonine kinases, Cdc7 (DDK) and CDK, leading to the assembly and activation of Cdc45/MCM2-7/GINS (CMG) helicases at the entry into the S phase and the formation of replisomes for bidirectional DNA synthesis. Biochemical and structural analyses of the recruitment of initiation or firing factors to the dhMCM2-7 for the formation of an active helicase and those of origin melting and DNA unwinding support the steric exclusion unwinding model of the CMG helicase.
Details
- Language :
- English
- ISSN :
- 20797737
- Volume :
- 13
- Issue :
- 8
- Database :
- Directory of Open Access Journals
- Journal :
- Biology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.6edabcb6f52543b6800ba6884e04339e
- Document Type :
- article
- Full Text :
- https://doi.org/10.3390/biology13080629