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The Outcomes and Quality of Pancreatic Islet Cells Isolated from Surgical Specimens for Research on Diabetes Mellitus

Authors :
Ju Yun Oh
Yang Hee Kim
Song Lee
Yu Na Lee
Han Se Go
Dae Wook Hwang
Ki Byung Song
Jae Hoon Lee
Woohyung Lee
Seongjun So
Eunju Kang
Eunsung Jun
In Kyong Shim
Song Cheol Kim
Source :
Cells, Vol 11, Iss 15, p 2335 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Isolating a large quantity of high-quality human islets is a prerequisite for diabetes research. Human islets are typically isolated from the pancreases of brain-dead donors, making research difficult due to low availability. Pancreas tissue discarded after surgical resection may be a good alternative source of islet cells. To test this hypothesis, we isolated islets from discarded surgical specimens and evaluated the islet yield and quality as well as islet cell preparations. Eighty-two segmental pancreases were processed using the Ricordi automated method, and islet yield and quality were investigated. The mean age of patients was 54.6, and the cohort included 32 diabetes patients. After purification, partially resected pancreases yielded an average of 59,593 ± 56,651 islet equivalents (IEQs) and 2546 IEQ/g of digested pancreas, with 71.5 ± 21% purity. Multivariate analysis revealed that diabetes (p = 0.0046) and the lobe used (p = 0.0156) significantly altered islet yield. Islets transplanted into diabetic mice displayed good viability and in vitro glucose responses, DNA/RNA quality, mitochondrial function, and glucose control, even though these results were dependent on islet quality. Isolated cells also maintained high viability and function even after cryopreservation. Our findings indicate that pancreatic tissue discarded after surgery can be a valuable source of islets for diabetes research.

Details

Language :
English
ISSN :
20734409
Volume :
11
Issue :
15
Database :
Directory of Open Access Journals
Journal :
Cells
Publication Type :
Academic Journal
Accession number :
edsdoj.6f2ab5ec2b54953b320d4db30e03b9e
Document Type :
article
Full Text :
https://doi.org/10.3390/cells11152335