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Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds

Authors :
Hisahiro Kai
Ena Akamatsu
Eri Torii
Hiroko Kodama
Chizuko Yukizaki
Isao Akagi
Hisatoshi Ino
Yoichi Sakakibara
Masahito Suiko
Ikuo Yamamoto
Akihiko Okayama
Kazuhiro Morishita
Hiroaki Kataoka
Koji Matsuno
Source :
Plants, Vol 3, Iss 1, Pp 18-26 (2013)
Publication Year :
2013
Publisher :
MDPI AG, 2013.

Abstract

In our previous report, an 80% ethanol bitter gourd seed extract (BGSE) was found to suppress proliferation of adult T-cell leukemia (ATL) cell lines. The present study aimed to identify the bioactive compounds from BGSE specific against ATL. From the result of an HPLC-MS analysis, α-eleostearic acid (α-ESA) was present in BGSE at 0.68% ± 0.0022% (±SD, n = 5). In the cell proliferation test, α-ESA potently suppressed proliferation of two ATL cell lines (ED and Su9T01; IC50 = 8.9 and 29.3 µM, respectively) more than several other octadecanoic acids. However, α-ESA moderately inhibited phytohemagglutinin-activated human peripheral blood mononuclear cells (PBMC; IC50 = 31.0 µM). These results suggest that BGSE-derived α-ESA has potential as a functional food constituent because of its activity against ATL, particularly against ED cells. Moreover, α-ESA might be effective for the prevention of moderate adverse effects of ATL on normal T cells.

Details

Language :
English
ISSN :
22237747
Volume :
3
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Plants
Publication Type :
Academic Journal
Accession number :
edsdoj.6f952e8abdf14ddb9dd66f8a1adaabdb
Document Type :
article
Full Text :
https://doi.org/10.3390/plants3010018