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The therapeutic effect of tanshinone IIA in mouse astrocytes after treatment with Angiostrongylus cantonensis fifth-stage larval excretory-secretory products

Authors :
Kuang-Yao Chen
Yi-Ju Chen
Chien-Ju Cheng
Kai-Yuan Jhan
Cheng-Hsun Chiu
Lian-Chen Wang
Source :
Journal of Microbiology, Immunology and Infection, Vol 56, Iss 4, Pp 853-862 (2023)
Publication Year :
2023
Publisher :
Elsevier, 2023.

Abstract

Background: Angiostrongylus cantonensis is an important food-borne zoonotic parasite that causes eosinophilic meningitis and meningoencephalitis in humans. Excretory-secretory products (ESPs) are valuable targets for studying host-parasite relationships. ESPs are composed of a variety of molecules that are used to penetrate defensive barriers and avoid immune attack of the host. Tanshinone IIA (TSIIA) is a vasoactive cardioprotective drug that is widely used in studies evaluating potential therapeutic mechanisms. In this study, we will evaluate the therapeutic effects of TSIIA in mouse astrocytes after A. cantonensis fifth-stage larvae (L5) ESPs treatment. Methods: Here, we examined the therapeutic effect of TSIIA by real-time qPCR, western blotting, activity assay, and cell viability assays. Results: First, the results showed that TSIIA can elevate cell viability in astrocytes after stimulation with ESPs. On the other hand, TSIIA downregulated the expression of apoptosis-related molecules. However, the expression of molecules related to antioxidant, autophagy, and endoplasmic reticulum stress was significantly increased. The results of antioxidant activation assays showed that the activities of superoxide dismutase (SOD), glutathione S-transferase (GST), and catalase were significantly increased. Finally, we found that cell apoptosis and oxidative stress were reduced in TSIIA-treated astrocytes by immunofluorescence staining. Conclusion: The findings from this study suggest that TSIIA can reduce cellular damage caused by A. cantonensis L5 ESPs in astrocytes and clarify the related molecular mechanisms.

Details

Language :
English
ISSN :
16841182
Volume :
56
Issue :
4
Database :
Directory of Open Access Journals
Journal :
Journal of Microbiology, Immunology and Infection
Publication Type :
Academic Journal
Accession number :
edsdoj.6ffad347e60f4b3e9fbb64cfbea21344
Document Type :
article
Full Text :
https://doi.org/10.1016/j.jmii.2023.04.007