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The Antioxidant Properties of Glucosinolates in Cardiac Cells Are Independent of H2S Signaling

Authors :
Félix Harvey
Boluwaji Aromokunola
Sabine Montaut
Guangdong Yang
Source :
International Journal of Molecular Sciences, Vol 25, Iss 2, p 696 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

The organic sulfur-containing compounds glucosinolates (GSLs) and the novel gasotransmitter H2S are known to have cardioprotective effects. This study investigated the antioxidant effects and H2S-releasing potential of three GSLs ((3E)-4-(methylsulfanyl)but-3-enyl GSL or glucoraphasatin, 4-hydroxybenzyl GSL or glucosinalbin, and (RS)-6-(methylsulfinyl)hexyl GSL or glucohesperin) in rat cardiac cells. It was found that all three GSLs had no effect on cardiac cell viability but were able to protect against H2O2-induced oxidative stress and cell death. NaHS, a H2S donor, also protected the cells from H2O2-stimulated oxidative stress and cell death. The GSLs alone or mixed with cysteine, N-acetylcysteine, glutathione, H2O2, iron and pyridoxal-5′-phosphate, or mouse liver lysates did not induce H2S release. The addition of GSLs also did not alter endogenous H2S levels in cardiac cells. H2O2 significantly induced cysteine oxidation in the cystathionine gamma-lyase (CSE) protein and inhibited the H2S production rate. In conclusion, this study found that the three tested GSLs protect cardiomyocytes from oxidative stress and cell death but independently of H2S signaling.

Details

Language :
English
ISSN :
14220067 and 16616596
Volume :
25
Issue :
2
Database :
Directory of Open Access Journals
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.714886b54ad24123945544a3c003d46d
Document Type :
article
Full Text :
https://doi.org/10.3390/ijms25020696