Inflammatory cytokines and apoptosis related proteins participate in the formation of deep vein thrombosis of lower extremity in rats

Objective To investigate the expression and regulation mechanism of IL-1β, TNF-α, Bcl-2 and BAX in rats with inferior vena cava thrombosis. Methods The rats were randomly divided into control group, inferior vena cava thrombus model group, mir-5189-3p inhibitor group and miR-5189-3p inhibitor NC group. Twenty-four hours later, the animals were killed by cervical vertebrae dislocation, blood samples were taken and tissues were collected; the level of IL-1β and TNF-α in blood samples were detected by ELISA; The morphology of inferior vena cava thrombus was microscopied with HE staining; The expression of Bcl-2 and BAX protein in inferior vena cava was detected by Western blot. Results The level of IL-1β and TNF-α in the model group was significantly higher than those in the control group (P＜0.05); the level of miR-5189-3p inhibitor group was significantly higher than that in the control group (P＜0.05); the level of miR-5189-3p inhibitor group was significantly higher than that in the model group (P＜0.05); the level of miR-5189-3p inhibitor negative control group was significantly lower than that in the model group (P＜0.05). In the model group and miR-5189-3p inhibitor group, red thrombus and mixed thrombus were found; the vessel showed inflammatory cell infiltration, and thrombus in the negative control group of miR-5189-3p inhibitor was obviously organized, with signs of blood flow recanalization. Bcl-2/BAX protein level in the model group was significantly lower than that in the control group (P＜0.05); miR-5189-3p inhibitor group was significantly lower than that in the model group (P＜0.05); while miR-5189-3p inhibitor in negative control group was significantly higher than that in the model group (P＜0.05). Conclusions miR-5189-3p can affect the formation of DVT by regulating the expression of inflammatory cytokines and apoptosis related proteins, which provides a new idea and basis for later research.