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Eradication of Krebs-2 primary ascites via a single-injection regimen of cyclophosphamide and double-stranded DNA
- Source :
- Вавиловский журнал генетики и селекции, Vol 20, Iss 5, Pp 716-722 (2016)
- Publication Year :
- 2016
- Publisher :
- Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders, 2016.
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Abstract
- Previously, we reported on the development of a therapeutic regimen allowing eradication of primary murine Krebs-2 ascites transplants. This protocol involved multiple injections of dsDNA preparations administered during the NER and HR phases of repair of interstrand DNA cross-links induced by prior cyclophosphamide treatments. Mice treated under this protocol frequently developed secondary ascites, which indicated that some tumor-inducing cancer stem cells could survive the treatment and caused relapse. Further, we observed that animals receiving multiple dsDNA injections developed pronounced systemic inflammatory response. This prompted us to develop a more straightforward treatment regimen based on the synergistic activity of cyclophosphamide and dsDNA preparations, which would allow complete eradication of established primary Krebs-2 ascites and also be less toxic for the treated animals. This protocol relies on a precisely timed single injection of dsDNA during the NER/HR transition period of each repair cycle. Under this protocol, 8-day remission of Krebs-2 engrafted mice was achieved, which was similar to the results of the multiple-injection treatment schedule. We observed an increase in the average life span of Krebs-2- transplanted mice on a single-injection regimen, which was consistent with reduced toxicity of such treatment.
Details
- Language :
- English, Russian
- ISSN :
- 25003259
- Volume :
- 20
- Issue :
- 5
- Database :
- Directory of Open Access Journals
- Journal :
- Вавиловский журнал генетики и селекции
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.72e755fdce694cac9febfbc60bf7e694
- Document Type :
- article
- Full Text :
- https://doi.org/10.18699/VJ16.161