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Understanding and Modulating Antibody Fine Specificity: Lessons from Combinatorial Biology

Authors :
Gertrudis Rojas
Source :
Antibodies, Vol 11, Iss 3, p 48 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Combinatorial biology methods such as phage and yeast display, suitable for the generation and screening of huge numbers of protein fragments and mutated variants, have been useful when dissecting the molecular details of the interactions between antibodies and their target antigens (mainly those of protein nature). The relevance of these studies goes far beyond the mere description of binding interfaces, as the information obtained has implications for the understanding of the chemistry of antibody–antigen binding reactions and the biological effects of antibodies. Further modification of the interactions through combinatorial methods to manipulate the key properties of antibodies (affinity and fine specificity) can result in the emergence of novel research tools and optimized therapeutics.

Details

Language :
English
ISSN :
20734468
Volume :
11
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Antibodies
Publication Type :
Academic Journal
Accession number :
edsdoj.73d733fd97934f44a03293275e5bf8f4
Document Type :
article
Full Text :
https://doi.org/10.3390/antib11030048