Back to Search Start Over

Mouse Sertoli Cells Inhibit Humoral-Based Immunity

Authors :
Rachel L. Washburn
Gurvinder Kaur
Jannette M. Dufour
Source :
International Journal of Molecular Sciences, Vol 23, Iss 21, p 12760 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Transplantation is used to treat many different diseases; however, without the use of immunosuppressants, which can be toxic to the patient, grafted tissue is rejected by the immune system. Humoral immune responses, particularly antibodies and complement, are significant components in rejection. Remarkably, Sertoli cells (SCs), immunoregulatory testicular cells, survive long-term after transplantation without immunosuppression. The objective of this study was to assess SC regulation of these humoral-based immune factors. Mouse SCs survived in vitro human complement (model of robust complement-mediated rejection) and survived in vivo as allografts with little-to-no antibody or complement fragment deposition. Microarray data and ELISA analyses identified at least 14 complement inhibitory proteins expressed by mouse SCs, which inhibit complement at multiple points. Interestingly, a mouse SC line (MSC-1), which was rejected by day 20 post transplantation, also survived in vitro human complement, showed limited deposition of antibodies and complement, and expressed complement inhibitors. Together this suggests that SC inhibition of complement-mediated killing is an important component of SC immune regulation. However, other mechanisms of SC immune modulation are also likely involved in SC graft survival. Identifying the mechanisms that SCs use to achieve extended survival as allografts could be utilized to improve graft survival.

Details

Language :
English
ISSN :
14220067 and 16616596
Volume :
23
Issue :
21
Database :
Directory of Open Access Journals
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.74192da7f3a400c81f1848059d97cbd
Document Type :
article
Full Text :
https://doi.org/10.3390/ijms232112760