Back to Search Start Over

Lactate Stimulates a Potential for Hypertrophy and Regeneration of Mouse Skeletal Muscle

Authors :
Yoshitaka Ohno
Koki Ando
Takafumi Ito
Yohei Suda
Yuki Matsui
Akiko Oyama
Hikari Kaneko
Shingo Yokoyama
Tatsuro Egawa
Katsumasa Goto
Source :
Nutrients, Vol 11, Iss 4, p 869 (2019)
Publication Year :
2019
Publisher :
MDPI AG, 2019.

Abstract

The effects of lactate on muscle mass and regeneration were investigated using mouse skeletal muscle tissue and cultured C2C12 cells. Male C57BL/6J mice were randomly divided into (1) control, (2) lactate (1 mol/L in distilled water, 8.9 mL/g body weight)-administered, (3) cardio toxin (CTX)-injected (CX), and (4) lactate-administered after CTX-injection (LX) groups. CTX was injected into right tibialis anterior (TA) muscle before the oral administration of sodium lactate (five days/week for two weeks) to the mice. Oral lactate administration increased the muscle weight and fiber cross-sectional area, and the population of Pax7-positive nuclei in mouse TA skeletal muscle. Oral administration of lactate also facilitated the recovery process of CTX-associated injured mouse TA muscle mass accompanied with a transient increase in the population of Pax7-positive nuclei. Mouse myoblast-derived C2C12 cells were differentiated for five days to form myotubes with or without lactate administration. C2C12 myotube formation with an increase in protein content, fiber diameter, length, and myo-nuclei was stimulated by lactate. These observations suggest that lactate may be a potential molecule to stimulate muscle hypertrophy and regeneration of mouse skeletal muscle via the activation of muscle satellite cells.

Details

Language :
English
ISSN :
20726643
Volume :
11
Issue :
4
Database :
Directory of Open Access Journals
Journal :
Nutrients
Publication Type :
Academic Journal
Accession number :
edsdoj.74383635144746a68136501148b28c89
Document Type :
article
Full Text :
https://doi.org/10.3390/nu11040869