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Pseudomonas aeruginosa Biofilm Dispersion by the Human Atrial Natriuretic Peptide

Authors :
Mélissande Louis
Thomas Clamens
Ali Tahrioui
Florie Desriac
Sophie Rodrigues
Thibaut Rosay
Nicholas Harmer
Suraya Diaz
Magalie Barreau
Pierre‐Jean Racine
Eric Kipnis
Teddy Grandjean
Julien Vieillard
Emeline Bouffartigues
Pierre Cornelis
Sylvie Chevalier
Marc G. J. Feuilloley
Olivier Lesouhaitier
Source :
Advanced Science, Vol 9, Iss 7, Pp n/a-n/a (2022)
Publication Year :
2022
Publisher :
Wiley, 2022.

Abstract

Abstract Pseudomonas aeruginosa biofilms cause chronic, antibiotic tolerant infections in wounds and lungs. Numerous recent studies demonstrate that bacteria can detect human communication compounds through specific sensor/receptor tools that modulate bacterial physiology. Consequently, interfering with these mechanisms offers an exciting opportunity to directly affect the infection process. It is shown that the human hormone Atrial Natriuretic Peptide (hANP) both prevents the formation of P. aeruginosa biofilms and strongly disperses established P. aeruginosa biofilms. This hANP action is dose‐dependent with a strong effect at low nanomolar concentrations and takes effect in 30–120 min. Furthermore, although hANP has no antimicrobial effect, it acts as an antibiotic adjuvant. hANP enhances the antibiofilm action of antibiotics with diverse modes of action, allowing almost full biofilm eradication. The hANP effect requires the presence of the P. aeruginosa sensor AmiC and the AmiR antiterminator regulator, indicating a specific mode of action. These data establish the activation of the ami pathway as a potential mechanism for P. aeruginosa biofilm dispersion. hANP appears to be devoid of toxicity, does not enhance bacterial pathogenicity, and acts synergistically with antibiotics. These data show that hANP is a promising powerful antibiofilm weapon against established P. aeruginosa biofilms in chronic infections.

Details

Language :
English
ISSN :
21983844
Volume :
9
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Advanced Science
Publication Type :
Academic Journal
Accession number :
edsdoj.7485e31d93094ff7b1bcfd7679edc5e3
Document Type :
article
Full Text :
https://doi.org/10.1002/advs.202103262