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An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay

Authors :
Francesca Costantini
Roald M. Tiggelaar
Riccardo Salvio
Marco Nardecchia
Stefan Schlautmann
Cesare Manetti
Han J. G. E. Gardeniers
Giampiero de Cesare
Domenico Caputo
Augusto Nascetti
Source :
Biosensors, Vol 7, Iss 4, p 58 (2017)
Publication Year :
2017
Publisher :
MDPI AG, 2017.

Abstract

A lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding. Thin film amorphous silicon photosensors have been fabricated on the sealed microfluidic substrate preventing the contamination of the micro-channels. The microfluidic network is then made accessible by opening inlets and outlets just prior to the use, ensuring the sterility of the device. The entire fabrication process relies on conventional photolithographic microfabrication techniques and is suitable for low-cost mass production of the device. The lab-on-chip system has been tested by implementing a chemiluminescent biochemical reaction. The inner channel walls of the microfluidic network are chemically functionalized with a layer of polymer brushes and horseradish peroxidase is immobilized into the coated channel. The results demonstrate the successful on-chip detection of hydrogen peroxide down to 18 μM by using luminol and 4-iodophenol as enhancer agent.

Details

Language :
English
ISSN :
20796374
Volume :
7
Issue :
4
Database :
Directory of Open Access Journals
Journal :
Biosensors
Publication Type :
Academic Journal
Accession number :
edsdoj.75742ba94bf542fbbf0e43d7b3746c08
Document Type :
article
Full Text :
https://doi.org/10.3390/bios7040058