Back to Search Start Over

A human fetal cerebellar map of the late second trimester reveals developmental molecular characteristics and abnormality in trisomy 21

Authors :
Hongmin Yu
Yun Liu
Fanqing Xu
Yuanyuan Fu
Ming Yang
Ling Ding
Yixuan Wu
Fuchou Tang
Jie Qiao
Lu Wen
Source :
Cell Reports, Vol 43, Iss 8, Pp 114586- (2024)
Publication Year :
2024
Publisher :
Elsevier, 2024.

Abstract

Summary: Our understanding of human fetal cerebellum development during the late second trimester, a critical period for the generation of astrocytes, oligodendrocytes, and unipolar brush cells (UBCs), remains limited. Here, we performed single-cell RNA sequencing (scRNA-seq) in human fetal cerebellum samples from gestational weeks (GWs) 18–25. We find that proliferating UBC progenitors distribute in the subventricular zone of the rhombic lip (RLSVZ) near white matter (WM), forming a layer structure. We also delineate two trajectories from astrogenic radial glia (ARGs) to Bergmann glial progenitors (BGPs) and recognize oligodendrogenic radial glia (ORGs) as one source of primitive oligodendrocyte progenitor cells (PriOPCs). Additionally, our scRNA-seq analysis of the trisomy 21 fetal cerebellum at this stage reveals abnormal upregulated genes in pathways such as the cell adhesion pathway and focal adhesion pathway, which potentially promote neuronal differentiation. Overall, our research provides valuable insights into normal and abnormal development of the human fetal cerebellum.

Details

Language :
English
ISSN :
22111247
Volume :
43
Issue :
8
Database :
Directory of Open Access Journals
Journal :
Cell Reports
Publication Type :
Academic Journal
Accession number :
edsdoj.757c32592fb347468065371cdee568ad
Document Type :
article
Full Text :
https://doi.org/10.1016/j.celrep.2024.114586