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Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs

Authors :
Kale Kundert
James E. Lucas
Kyle E. Watters
Christof Fellmann
Andrew H. Ng
Benjamin M. Heineike
Christina M. Fitzsimmons
Benjamin L. Oakes
Jiuxin Qu
Neha Prasad
Oren S. Rosenberg
David F. Savage
Hana El-Samad
Jennifer A. Doudna
Tanja Kortemme
Source :
Nature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
Publication Year :
2019
Publisher :
Nature Portfolio, 2019.

Abstract

Control of CRISPR-Cas9 activity allows for fine-tuning of editing and gene expression. Here the authors use gRNAs modified with RNA aptamers to enable small molecule control in bacterial systems.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
10
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.75a198d8f2ba47a985055e98863b4ffd
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-019-09985-2