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Application of metagenomic next-generation sequencing in the clinical diagnosis of infectious diseases after allo-HSCT: a single-center analysis

Authors :
Hailong Yuan
Xiaolu Ma
Jianli Xu
Peng Han
Guanhua Rao
Gang Chen
Kaile Zhang
Ruixue Yang
Chuixia Han
Ming Jiang
Source :
BMC Infectious Diseases, Vol 24, Iss 1, Pp 1-13 (2024)
Publication Year :
2024
Publisher :
BMC, 2024.

Abstract

Abstract Background We investigated the value of metagenomic next-generation sequencing (mNGS) in diagnosing infectious diseases in patients receiving allogeneic hematopoietic stem cell transplantation (allo-HSCT). Methods Fifty-four patients who had fever following allo-HSCT from October 2019 to February 2022 were enrolled. Conventional microbiological tests (CMTs) and mNGS, along with imaging and clinical manifestations, were used to diagnose infection following allo-HSCT. The clinical diagnostic value of mNGS was evaluated. Results A total of 61 mNGS tests were performed, resulting in the diagnosis of 46 cases of infectious diseases. Among these cases, there were 22 cases of viral infection, 13 cases of fungal infection, and 11 cases of bacterial infection. Moreover, 27 cases (58.7%) were classified as bloodstream infections, 15 (32.6%) as respiratory infections, 2 (4.3%) as digestive system infections, and 2 (4.3%) as central nervous system infections. Additionally, there were 8 cases with non-infectious diseases (8/54, 14.81%), including 2 cases of interstitial pneumonia, 2 cases of bronchiolitis obliterans, 2 cases of engraftment syndrome, and 2 cases of acute graft-versus-host disease. The positive detection rates of mNGS and CMT were 88.9% and 33.3%, respectively, with significant differences (P

Details

Language :
English
ISSN :
14712334
Volume :
24
Issue :
1
Database :
Directory of Open Access Journals
Journal :
BMC Infectious Diseases
Publication Type :
Academic Journal
Accession number :
edsdoj.774e26dbf50c49ff9f443ac4a551b703
Document Type :
article
Full Text :
https://doi.org/10.1186/s12879-024-09153-y