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Position of UNC-13 in the active zone regulates synaptic vesicle release probability and release kinetics
- Source :
- eLife, Vol 2 (2013)
- Publication Year :
- 2013
- Publisher :
- eLife Sciences Publications Ltd, 2013.
-
Abstract
- The presynaptic active zone proteins UNC-13/Munc13s are essential for synaptic vesicle (SV) exocytosis by directly interacting with SV fusion apparatus. An open question is how their association with active zones, hence their position to Ca2+ entry sites, regulates SV release. The N-termini of major UNC-13/Munc13 isoforms contain a non-calcium binding C2A domain that mediates protein homo- or hetero-meric interactions. Here, we show that the C2A domain of Caenorhabditis elegans UNC-13 regulates release probability of evoked release and its precise active zone localization. Kinetics analysis of SV release supports that the proximity of UNC-13 to Ca2+ entry sites, mediated by the C2A-domain containing N-terminus, is critical for accelerating neurotransmitter release. Additionally, the C2A domain is specifically required for spontaneous release. These data reveal multiple roles of UNC-13 C2A domain, and suggest that spontaneous release and the fast phase of evoked release may involve a common pool of SVs at the active zone.
Details
- Language :
- English
- ISSN :
- 2050084X
- Volume :
- 2
- Database :
- Directory of Open Access Journals
- Journal :
- eLife
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.786074d89c894632a8ee4d51c7bbaaed
- Document Type :
- article
- Full Text :
- https://doi.org/10.7554/eLife.01180