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Development of an antibody fragment that stabilizes GPCR/G-protein complexes

Authors :
Shoji Maeda
Antoine Koehl
Hugues Matile
Hongli Hu
Daniel Hilger
Gebhard F. X. Schertler
Aashish Manglik
Georgios Skiniotis
Roger J. P. Dawson
Brian K. Kobilka
Source :
Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
Publication Year :
2018
Publisher :
Nature Portfolio, 2018.

Abstract

The determination of high resolution structures of G protein coupled receptors (GPCRs) in complex with heterotrimeric G proteins is challenging. Here authors develop an antibody fragment, mAB16, which stabilizes GPCR/G-protein complexes and facilitates the application of high resolution cryo-EM.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723 and 78767849
Volume :
9
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.78767849a46d4536a969df36dd817784
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-018-06002-w