Back to Search
Start Over
Molecular Typing of Australian Scedosporium Isolates Showing Genetic Variability and Numerous S. aurantiacum
- Source :
- Emerging Infectious Diseases, Vol 14, Iss 2, Pp 282-290 (2008)
- Publication Year :
- 2008
- Publisher :
- Centers for Disease Control and Prevention, 2008.
-
Abstract
- One hundred clinical isolates from a prospective nationwide study of scedosporiosis in Australia (2003–2005) and 46 additional isolates were genotyped by internal transcribed spacer–restriction fragment length polymorphism (ITS-RFLP) analysis, ITS sequencing, and M13 PCR fingerprinting. ITS-RFLP and PCR fingerprinting identified 3 distinct genetic groups. The first group corresponded to Scedosporium prolificans (n = 83), and the other 2 comprised isolates previously identified as S. apiospermum: one of these corresponded to S. apiospermum (n = 33) and the other to the newly described species S. aurantiacum (n = 30). Intraspecies variation was highest for S. apiospermum (58%), followed by S. prolificans (45%) and S. aurantiacum (28%) as determined by PCR fingerprinting. ITS sequence variation of 2.2% was observed among S. apiospermum isolates. No correlation was found between genotype of strains and their geographic origin, body site from which they were cultured, or colonization versus invasive disease. Twelve S. prolificans isolates from 2 suspected case clusters were examined by amplified fragment length polymorphism analysis. No specific clusters were confirmed.
Details
- Language :
- English
- ISSN :
- 10806040 and 10806059
- Volume :
- 14
- Issue :
- 2
- Database :
- Directory of Open Access Journals
- Journal :
- Emerging Infectious Diseases
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.78b69f977ba14e3eb3f7ea6fbf249c0f
- Document Type :
- article
- Full Text :
- https://doi.org/10.3201/eid1402.070920