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Spectrum and Density of Gamma and X-ray Induced Mutations in a Non-Model Rice Cultivar

Authors :
Joanna Jankowicz-Cieslak
Bernhard J. Hofinger
Luka Jarc
Sini Junttila
Bence Galik
Attila Gyenesei
Ivan L. Ingelbrecht
Bradley J. Till
Source :
Plants, Vol 11, Iss 23, p 3232 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Physical mutagens are a powerful tool used for genetic research and breeding for over eight decades. Yet, when compared to chemical mutagens, data sets on the effect of different mutagens and dosages on the spectrum and density of induced mutations remain lacking. To address this, we investigated the landscape of mutations induced by gamma and X-ray radiation in the most widely cultivated crop species: rice. A mutant population of a tropical upland rice, Oryza sativa L., was generated and propagated via self-fertilization for seven generations. Five dosages ranging from 75 Gy to 600 Gy in both X-ray and gamma-irradiated material were applied. In the process of a forward genetic screens, 11 unique rice mutant lines showing phenotypic variation were selected for mutation analysis via whole-genome sequencing. Thousands of candidate mutations were recovered in each mutant with single base substitutions being the most common, followed by small indels and structural variants. Higher dosages resulted in a higher accumulation of mutations in gamma-irradiated material, but not in X-ray-treated plants. The in vivo role of all annotated rice genes is yet to be directly investigated. The ability to induce a high density of single nucleotide and structural variants through mutagenesis will likely remain an important approach for functional genomics and breeding.

Details

Language :
English
ISSN :
22237747
Volume :
11
Issue :
23
Database :
Directory of Open Access Journals
Journal :
Plants
Publication Type :
Academic Journal
Accession number :
edsdoj.78db8fc6bba4dab91c1ba6e80c7d934
Document Type :
article
Full Text :
https://doi.org/10.3390/plants11233232