Back to Search
Start Over
Optimized PCR with sequence specific primers (PCR-SSP) for fast and efficient determination of Interleukin-6 Promoter -597/-572/-174Haplotypes
- Source :
- BMC Research Notes, Vol 2, Iss 1, p 245 (2009)
- Publication Year :
- 2009
- Publisher :
- BMC, 2009.
-
Abstract
- Abstract Background Interleukin-6 (IL-6) promoter polymorphisms at positions -597(G→A), -572(G→C) and -174(G→C) were shown to have a clinical impact on different major diseases. At present PCR-SSP protocols for IL-6 -597/-572/-174haplotyping are elaborate and require large amounts of genomic DNA. Findings We describe an improved typing technique requiring a decreased number of PCR-reactions and a reduced PCR-runtime due to optimized PCR-conditions. Conclusion This enables a fast and efficient determination of IL-6 -597/-572/-174haplotypes in clinical diagnosis and further evaluation of IL-6 promoter polymorphisms in larger patient cohorts.
- Subjects :
- Medicine
Biology (General)
QH301-705.5
Science (General)
Q1-390
Subjects
Details
- Language :
- English
- ISSN :
- 17560500
- Volume :
- 2
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- BMC Research Notes
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.79b4475f3445f79749b78916c278f0
- Document Type :
- article
- Full Text :
- https://doi.org/10.1186/1756-0500-2-245