Back to Search
Start Over
Development of biomimetic enzyme-linked immunosorbent assay based on molecular imprinting technique for semicarbazide detection
Development of biomimetic enzyme-linked immunosorbent assay based on molecular imprinting technique for semicarbazide detection
- Source :
- Food and Agricultural Immunology, Vol 31, Iss 1, Pp 17-32 (2020)
- Publication Year :
- 2020
- Publisher :
- Taylor & Francis Group, 2020.
-
Abstract
- Herein, we present a direct competitive biomimetic enzyme-linked immunosorbent assay (BELISA) method for the determination of semicarbazide (SEM) using a molecularly imprinted polymer (MIP) film as the bionic antibody. The best monomer (methacrylic acid) and molar ratio of monomer to template (4:1) were determined with the aid of computational simulation methods based on density functional theory (DFT). The MIP film was characterised by scanning electron microscopy and adsorption experiments. The results showed that the imprinted film was successfully synthesised with good adsorption performance. Under optimal conditions, the calibration curve was linear in the range of 1.0–1.0×104 ng/mL (R2 = 0.9863). The detection limit (IC15) and sensitivity (IC50) were 1.0 and 85.7 ng/mL, respectively. This BELISA method was further applied to the analysis of SEM in food samples; recoveries ranged from 86.1% to 90.8%. These observations indicated that the developed method could be adapted for SEM quantification in food.
Details
- Language :
- English
- ISSN :
- 09540105 and 14653443
- Volume :
- 31
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Food and Agricultural Immunology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.7b1656ec20f942a7a8e9b960f0bb256b
- Document Type :
- article
- Full Text :
- https://doi.org/10.1080/09540105.2019.1692789