Genetic Relationships Among Medicago sativa L. Clones Commonly Grown in Central Anatolia

Amplified fragment length polymorphism AFLP markers were used to investigate genotypic variability among 34 alfalfa clones M. sativa using 15 primer combinations with restriction enzymes EcoRI, PstI and MseI. 34 unique AFLP fragments were observed. The 15 primer pairs produced a total of 1002 fragments of which 460 were polymorphic. The number of polymorphic fragments detected per primer combination ranged 7 to 67. Furthermore, 22 clone-specific markers were also detected in the 13 clones. Data analysis was performed with NTSYSpc version 2.1 software. Genetic distance values ranged 5.9374 to 1.1453. Fifteen clones which showed the highest genetic variation were selected for producing synthetic variety of Alfalfa