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Estrogen receptor alpha (ERα) regulates PARN-mediated nuclear deadenylation and gene expression in breast cancer cells

Authors :
Sophia Varriano
Amy Yu
Yu Qing Xu
Devorah M. Natelson
Anthony Ramadei
Frida E. Kleiman
Source :
RNA Biology, Vol 21, Iss 1, Pp 1090-1099 (2024)
Publication Year :
2024
Publisher :
Taylor & Francis Group, 2024.

Abstract

The estrogen signalling pathway is highly dynamic and primarily mediated by estrogen receptors (ERs) that transcriptionally regulate the expression of target genes. While transcriptional functions of ERs have been widely studied, their roles in RNA biology have not been extensively explored. Here, we reveal a novel biological role of ER alpha (ERα) in mRNA 3’ end processing in breast cancer cells, providing an alternative mechanism in regulating gene expression at the post-transcriptional level. We show that ERα activates poly(A) specific ribonuclease (PARN) deadenylase using in vitro assays, and that this activation is further increased by tumour suppressor p53, a factor involved in mRNA processing. Consistent with this, we confirm ERα-mediated activation of nuclear deadenylation by PARN in samples from MCF7 and T47D breast cancer cells that vary in expression of ERα and p53. We further show that ERα can form complex(es) with PARN and p53. Lastly, we identify and validate expression of common mRNA targets of ERα and PARN known to be involved in cell invasion, metastasis and angiogenesis, supporting the functional overlap of these factors in regulating gene expression in a transactivation-independent manner. Together, these results show a new regulatory mechanism by which ERα regulates mRNA processing and gene expression post-transcriptionally, highlighting its contribution to unique transcriptomic profiles and breast cancer progression.

Details

Language :
English
ISSN :
15476286 and 15558584
Volume :
21
Issue :
1
Database :
Directory of Open Access Journals
Journal :
RNA Biology
Publication Type :
Academic Journal
Accession number :
edsdoj.7d1a054dec2749678b78a2be4e5caf14
Document Type :
article
Full Text :
https://doi.org/10.1080/15476286.2024.2413821