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Regulation of Microcystin-LR-Induced DNA Damage by miR-451a in HL7702 Cells

Authors :
Lv Chen
Shu Yang
Cong Wen
Shuilin Zheng
Yue Yang
Xiangling Feng
Jihua Chen
Dan Luo
Ran Liu
Fei Yang
Source :
Toxins, Vol 11, Iss 3, p 164 (2019)
Publication Year :
2019
Publisher :
MDPI AG, 2019.

Abstract

Microcystin-LR is a cyclic heptapeptide hepatotoxin produced by harmful cyanobacteria. A panel of microRNAs containing miR-451a were found to be significantly changed in normal human liver cells HL7702 after exposure to microcystin-LR (MC-LR) in our previous study. However, the functions of miR-451a in hepatotoxicity induced by MC-LR remained unclear. The study aimed to investigate the impacts of miR-451a in HL7702 cells following treatment with 5 or 10 μM MC-LR. The comet assay indicated that MC-LR can influence Olive tail moment (OTM) in HL7702 cells. Furthermore, increase of miR-451a significantly repressed DNA damage and the protein expression level of γ-H2AX induced by MC-LR. Moreover, over-expression of miR-451a inhibited the expression level of p-AKT1 protein in cells following treatment by MC-LR. These results showed that miR-451a may protect from MC-LR-induced DNA damage by down-regulating the expression of p-AKT1, which provides new clues for the diagnosis and therapy policies for liver damage induced by MC-LR.

Details

Language :
English
ISSN :
20726651
Volume :
11
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Toxins
Publication Type :
Academic Journal
Accession number :
edsdoj.7df0165df16d444eb83b6dc45e72019d
Document Type :
article
Full Text :
https://doi.org/10.3390/toxins11030164