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SpheroMold: modernizing the hanging drop method for spheroid culture
- Source :
- Frontiers in Drug Delivery, Vol 4 (2024)
- Publication Year :
- 2024
- Publisher :
- Frontiers Media S.A., 2024.
-
Abstract
- The hanging drop method is a cost-effective approach for 3D spheroid culture. However, obtaining numerous spheroids in a limited area becomes challenging due to the risk of droplet coalescence, primarly during Petri dish handling. In this study, we describe a general method to fabricate a 3D printing-based support called SpheroMold that facilitates Petri dish handling and enhances spheroid production per unit area. As a proof-of-concept, we designed a digital negative mold which comprised 37 pegs within a 13.52 cm2 area, and then printed it using stereolithography; the density of pegs can be adjusted according to user requirements. The SpheroMold was created by pouring the base and curing agent (10:1) (Sylgard® 184 silicone) into the mold, curing it at 80°C, and then attaching it to the lid of a Petri dish. Our SpheroMold effectively prevented droplet coalescence during Petri dish inversion, enabling the production of numerous 3D spheroids while simplifying manipulation. Unlike conventional techniques, our design also facilitated a larger volume of culture medium per drop compared to a standard Petri dish, potentially decreasing the necessity for frequent medium exchange to sustain cellular health and reducing labor intensity.
Details
- Language :
- English
- ISSN :
- 26740850
- Volume :
- 4
- Database :
- Directory of Open Access Journals
- Journal :
- Frontiers in Drug Delivery
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.7f2c63c045e44fa1acdc43ef41f42002
- Document Type :
- article
- Full Text :
- https://doi.org/10.3389/fddev.2024.1397153