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IL-1α Up-Regulates IL-6 Expression in Bovine Granulosa Cells via MAPKs and NF-κB Signaling Pathways
- Source :
- Cellular Physiology and Biochemistry, Vol 41, Iss 1, Pp 265-273 (2017)
- Publication Year :
- 2017
- Publisher :
- Cell Physiol Biochem Press GmbH & Co KG, 2017.
-
Abstract
- Background/Aims: IL-6 is one of the main cytokines in regulating ovarian follicular development and ovulation. However, the factors that regulate IL-6 expression in follicles are still unclear. The aim of this study was to elucidate the mechanisms underlying the effect of IL-1α on IL-6 expression in granulosa cells. Methods: IL-6 expression after IL-1α with/without inhibitors treatment was analyzed by RT-qPCR and ELISA. The phosphorylation of proteins induced by IL-1α was analyzed by western blot. The intracellular cAMP level was assayed by immunoassay kit. Results: IL-1α has a dose-dependent effect on IL-6 expression in granulosa cells. This promoting effect can be significantly attenuated by Erk, c-Jun, p38 and IκB proteins inhibitors, respectively. Moreover, the phosphorylation levels of Erk, c-Jun, p38 and IκBα proteins were significantly increased after IL-1α treatment. In addition, we also found that IL-1α not only reversed the cAMP attenuated IL-6 expression, but also increased IL-1α mRNA expression in granulosa cells. Conclusion: The regulation of IL-1α on IL-6 expression is mediated by activation of MAPKs and NF-κB signaling pathways. Moreover,IL-1α may regulate the ovulation-related genes expression in granulosa cells by an autocrine and/or paracrine manner.
Details
- Language :
- English
- ISSN :
- 10158987 and 14219778
- Volume :
- 41
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Cellular Physiology and Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.7fc8b4222534d5b8284175456409b70
- Document Type :
- article
- Full Text :
- https://doi.org/10.1159/000456091