Examining the Effect of Inhibition of Lysyl oxidase on Involved Genes in Hypertrophy Following One Period of Resistance Training in Wistar Rats

Abstract Background: Recent Research has pointed to the involvement of lysyl oxidase (LOX) in the muscle development. Despite the fact, there is currently no direct evidence that lox is involved in the myogenic factors and exercise-induced hypertrophy. Therefore, the purpose of this study was to investigate the effect of lysyl oxidase inhibition on exercise-induced hypertrophy, as well as the gene expression of MyoD1, myogenin, TGF-β and LOX in FHL muscle of wistar rats. Materials and Methods: In this experimental research, 32 male Wister rats with an average weight of 220±15 were divided into four groups: resistance training, normal control, treated control and treated resistance training. Rats performed a resistance training for 8 weeks in which animals climbed a vertical ladder of 1¬m and inclined at 85° with weights attached to their tails. In order to inhibit lysyl oxidase, treatment group rats received daily injection of intraperitoneal β-aminopropionitrile (120 mg/kg/day). FHL muscle was extracted to measure relative muscle weight, as well as genes expression of MyoD1, myogenin, TGF-β and LOX by real time-PCR. Results: Relative weight of FHL muscle was decreased significantly in the treated groups with BAPN, compared with normal groups (p