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Construction and immune evaluation of the recombinant duck adenovirus type 3 delivering capsid protein VP1 of the type 1 duck hepatitis virus
- Source :
- Poultry Science, Vol 102, Iss 12, Pp 103117- (2023)
- Publication Year :
- 2023
- Publisher :
- Elsevier, 2023.
-
Abstract
- ABSTRACT: Adenovirus serves as an excellent viral vector and is employed in vector vaccine research. Duck hepatitis A virus type 1 (DHAV1) and duck adenovirus type 3 (DAdV3) cause significant economic losses in the Chinese duck industry. In this study, we found an excellent exogenous gene insertion site in DAdV3 genome of CH-GD-12-2014 strain, within 3 intergenic regions (IGR). Subsequently, we generated a recombinant duck adenovirus named rDAdV3-VP1-188, which exhibits excellent replication characteristics and immunogenicity of DAdV3 and DHAV1. Animal experiments showed that rDAdV3-VP1-188 can provide 100% protection against the DAdV3 and 80% protection against DHAV1. These results showed that rDAdV3-VP1-188 could induce protection against DAdV3 and DHAV1 in ducks, thus indicating the feasibility of DAdV3 as a vector for the development of avian vector vaccines. These insights contribute to the further development of DAdV3 vectors and other adenovirus vectors.
Details
- Language :
- English
- ISSN :
- 00325791
- Volume :
- 102
- Issue :
- 12
- Database :
- Directory of Open Access Journals
- Journal :
- Poultry Science
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.809bb83fa074bb5990b3b9317463495
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.psj.2023.103117