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RNA aptamers selected against yeast cells inhibit Candida albicans biofilm formation in vitro

Authors :
Boy M. Bachtiar
Chatchawan Srisawat
Endang W. Bachtiar
Source :
MicrobiologyOpen, Vol 8, Iss 8, Pp n/a-n/a (2019)
Publication Year :
2019
Publisher :
Wiley, 2019.

Abstract

Abstract Aptamers that bind live bacterial cells have been widely investigated, but their potential to inhibit Candida albicans biofilm formation needs to be further explored. The aims of this study were to evaluate the binding of C. albicans to RNA aptamers and to examine the potential of aptamers to inhibit C. albicans biofilm formation in vitro. In this study, RNA aptamers selected against yeast cells of C. albicans ATCC 10231 were developed using the systematic evolution of ligands by exponential enrichment (SELEX) technique. The binding affinity of the resulting aptamers was then determined by an aptamer‐linked immobilized sorbent assay (ALISA), and a colorimetric (MTT) assay was used to measure the metabolic activity of Candida biofilms. After 11 rounds of SELEX, two candidate aptamers, Ca‐apt‐1 and Ca‐apt‐12, were identified. The Ca‐apt‐1 aptamer also recognized C. albicans isolated from clinical specimens but did not recognize other oral microorganisms (i.e., Streptococcus mutans and Saccharomyces cerevisiae). The ALISA results showed that the binding affinity of these aptamers was comparable to that of an anti‐C. albicans monoclonal antibody. In addition, Ca‐apt‐1 could inhibit biofilm and hyphal formation of C. albicans in vitro, as demonstrated using biofilm assays. This study shows that RNA aptamers could potentially be used in diagnostic and therapeutic applications for C. albicans‐related disease in the future.

Details

Language :
English
ISSN :
20458827
Volume :
8
Issue :
8
Database :
Directory of Open Access Journals
Journal :
MicrobiologyOpen
Publication Type :
Academic Journal
Accession number :
edsdoj.821ee71e89754dfaa9ae9617d822926e
Document Type :
article
Full Text :
https://doi.org/10.1002/mbo3.812