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PiR-hsa-23533 promotes malignancy in head and neck squamous cell carcinoma via USP7
- Source :
- Translational Oncology, Vol 45, Iss , Pp 101990- (2024)
- Publication Year :
- 2024
- Publisher :
- Elsevier, 2024.
-
Abstract
- Background: With regard to head and neck squamous cell carcinoma (HNSCC), its occurrence and advancement are controlled by genetic and epigenetic anomalies. PIWI-interacting RNAs (piRNAs) are recognized with significance in tumor, but the precise molecular mechanisms of piRNAs in HNSCC largely remain undisclosed. Methods: Differentially expressed piRNAs were identified by RNA sequencing. The expression of piR-hsa-23533 was evaluated using quantitative real-time PCR and RNA in situ hybridization. The impacts of piR-hsa-23533 on the proliferation and apoptosis of HNSCC cells were investigated by a series of in vitro and in vivo assays. Results: piR-hsa-23533 exhibits upregulation within HNSCC cells and tissues. Besides, piR-hsa-23533 overexpression promotes proliferation while inhibiting apoptosis in vitro and in vivo, while piR-hsa-23533 silencing has an opposite function. From the mechanistic perspective, piR-hsa-23533 can bind to Ubiquitin-specific protease 7 (USP7), as shown through RNA pull-down and RNA immunoprecipitation assays, promoting USP7 mRNA and protein expression. Conclusions: These findings highlight the functional importance of piR-hsa-23533 in HNSCC and may assist in the development of anti-HNSCC therapeutic target.
Details
- Language :
- English
- ISSN :
- 19365233
- Volume :
- 45
- Issue :
- 101990-
- Database :
- Directory of Open Access Journals
- Journal :
- Translational Oncology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.8255c44602514f10b3f3494f060c4e85
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.tranon.2024.101990