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The Stability of Analytes of Ionized Magnesium Concentration and Its Reference Range in Healthy Volunteers

Authors :
Juhaina Salim Al-Maqbali
Abdullah M. Al Alawi
Zubaida Al-Falahi
Henrik Falhammar
Ibrahim Al-Zakwani
Mohammed Al Za’abi
Source :
Biomedicines, Vol 11, Iss 9, p 2539 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

This study aimed to determine the stability of refrigerated analytes of iMg concentration at different time intervals and to establish iMg reference range in a cohort of healthy Omani volunteers (≥18 years). The concentrations of iMg were measured using the direct ion-selective electrode technique. Pearson’s and Lin’s concordance correlation coefficients along with the Bland–Altman plot were used to assess the levels of agreement between iMg concentrations of fresh and refrigerated blood samples at different time intervals. The study included 167 volunteers (51% females) with a median age of 21 (range: 20–25) years. The median, 2.5th, and 97.5th percentiles for fresh iMg reference ranges were 0.55, 0.47, and 0.68 mmol/L, respectively. The overall agreement between the fresh and refrigerated iMg concentrations was poor (rho-c = 0.51; p < 0.001). However, according to Altman’s definition, iMg concentrations of the refrigerated samples for a period of ≤1 h had an excellent correlation with the fresh iMg concentrations (Lin’s rho-c = 0.80), with a small average bias difference of 0.009 (95%CI; −0.025–0.043). A cut-off refrigeration period within ≤1 h at 2–8 °C can be considered an alternate time frame for the gold standard measurement (fresh or within 0.5 h).

Details

Language :
English
ISSN :
22279059
Volume :
11
Issue :
9
Database :
Directory of Open Access Journals
Journal :
Biomedicines
Publication Type :
Academic Journal
Accession number :
edsdoj.837a0413afb8461d813fdf8e199ad76e
Document Type :
article
Full Text :
https://doi.org/10.3390/biomedicines11092539