Back to Search Start Over

OsCCRL1 is Essential for Phenylpropanoid Metabolism in Rice Anthers

Authors :
Lisha Zhang
Lintao Zheng
Jingwen Wu
Yang Liu
Weichi Liu
Guanghua He
Nan Wang
Source :
Rice, Vol 16, Iss 1, Pp 1-20 (2023)
Publication Year :
2023
Publisher :
SpringerOpen, 2023.

Abstract

Abstract Phenylpropanoid metabolism and timely tapetal degradation are essential for anther and pollen development, but the underlying mechanisms are unclear. In the current study, to investigate this, we identified and analyzed the male-sterile mutant, osccrl1 (cinnamoyl coA reductase-like 1), which exhibited delayed tapetal programmed cell death (PCD) and defective mature pollen. Map-based cloning, genetic complementation, and gene knockout revealed that OsCCRL1 corresponds to the gene LOC_Os09g32020.2, a member of SDR (short-chain dehydrogenase/reductase) family enzyme. OsCCRL1 was preferentially expressed in the tapetal cells and microspores, and localized to the nucleus and cytoplasm in both rice protoplasts and Nicotiana benthamiana leaves. The osccrl1 mutant exhibited reduced CCRs enzyme activity, less lignin accumulation, delayed tapetum degradation, and disrupted phenylpropanoid metabolism. Furthermore, an R2R3 MYB transcription factor OsMYB103/OsMYB80/OsMS188/BM1, involved in tapetum and pollen development, regulates the expression of OsCCRL1. Finally, the osmyb103 osccrl1 double mutants, exhibited the same phenotype as the osmyb103 single mutant, further indicating that OsMYB103/OsMYB80/OsMS188/BM1 functions upstream of OsCCRL1. These findings help to clarify the role of phenylpropanoid metabolism in male sterility and the regulatory network underlying the tapetum degradation.

Details

Language :
English
ISSN :
19398425 and 19398433
Volume :
16
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Rice
Publication Type :
Academic Journal
Accession number :
edsdoj.84000e806ac4be182b95f1a840fed6a
Document Type :
article
Full Text :
https://doi.org/10.1186/s12284-023-00628-1