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Visualizing Efficacy of Antineoplastic Drug via Ratiometric Near-Infrared Fluorescence Imaging of Tumor-Associated Macrophage-Specific Nitric Oxide
- Source :
- Chemical & Biomedical Imaging, Vol 1, Iss 4, Pp 372-379 (2023)
- Publication Year :
- 2023
- Publisher :
- American Chemical Society, 2023.
-
Abstract
- Elevated nitric oxide (NO) within tumor-associated macrophages (TAMs) suggests a reduction of TAM-mediated tumoral immune tolerance. This cellular event could be a reliable indicator for efficacy evaluation of antineoplastic drugs. However, a suitable method for TAM-specific NO measurement is still lacking. In this work, a simple and fast efficacy evaluation method for antineoplastic drugs is established based on a ratiometric TAM-specific NO near-infrared (NIR) fluorescence probe TAM-Cy-NO. Molecular fluorescence probe Cy-NO for NO response was encapsulated in the TAM-targeting peptide (M2pep)-functionalized liposome to construct TAM-Cy-NO. After TAM enters through M2pep, Cy-NO reacts with NO specifically, resulting in a dose-dependent ratiometric fluorescence signal (I610/I815) change manner. Utilizing this strategy, we observed that PLX-3397, metformin, and ibrutinib triggered NO generation within TAM greater than that with sorafenib. Notably, metformin and ibrutinib promoted TNF-α and reduced PD-L1 expressions, which suggest reductions of TAM-mediated immunosuppression. As expected, these drugs delayed tumor progression in mice. This method provides a promising efficacy evaluation strategy for rapid screening of antineoplastic drugs.
- Subjects :
- Medical technology
R855-855.5
Chemistry
QD1-999
Subjects
Details
- Language :
- English
- ISSN :
- 28323637
- Volume :
- 1
- Issue :
- 4
- Database :
- Directory of Open Access Journals
- Journal :
- Chemical & Biomedical Imaging
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.8424efc934a541b1b2835f30cda40298
- Document Type :
- article
- Full Text :
- https://doi.org/10.1021/cbmi.3c00016