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Comparison of haemoglobin concentration measurements using HemoCue-301 and Sysmex XN-Series 1500: A survey among anaemic Gambian infants aged 6-12 months.

Authors :
Mamadou Bah
Hans Verhoef
Abdou Camara
Morris Nden Ngom
Demba Jallow
Kebba Bajo
Foday Bah
Maarten Pleij
Maaike Klappe
Alasana Saidykhan
Emmanuel Okoh
Abdoulie Bah
Carla Cerami
Source :
PLoS ONE, Vol 19, Iss 11, p e0310577 (2024)
Publication Year :
2024
Publisher :
Public Library of Science (PLoS), 2024.

Abstract

BackgroundIn low-income countries, point-of-care photometers are used in the screening and management of anaemia in individuals, but also in the assessment of population iron status when evaluating efficacy of intervention studies or public health interventions.AimsWe aimed to evaluate the accuracy of a commonly used photometer, HemoCue-301, in determining haemoglobin concentration among anaemic children aged 6-12 months in a field setting in rural Africa.MethodsThis report concerns a secondary analysis of data from Gambian infants being screened for an ongoing randomized controlled trial. In those found to be anaemic by HemoCue-301, haemoglobin concentration was measured by Sysmex XN-1500, an automated haematology analyser that was used as a reference. Passing-Bablok regression analysis was used to estimate the regression constant (systematic deviation between two measurement methods that remain consistent across the range of measurements) and proportional bias (systematic deviation between two measurement methods that change in magnitude relative to the value being measured).ResultsAnalysis was based on 227 participants. There was strong evidence of absolute bias among moderately anaemic participants (haemoglobin concentration at 8.0g/dL) (absolute bias: 1.12g/dL; 95% CI: 0.91 to 1.37g/dL; proportional bias: 14.0%; 95% CI: 11.4% to 17.1%) in haemoglobin concentrations measured by HemoCue-301 compared to those measured by Sysmex XN-Series1500. Bias was marginal at haemoglobin concentration of 11.0g/dL (absolute bias: -0.08g/dL; 95% CI: -0.18 to 0.07g/dL; proportional bias: -7.3%; 95% CI: -6.5% to 0.6%).ConclusionHaemoglobin measurements by HemoCue-301 seem substantially biased in participants with haemoglobin less than 8.0g/dL.

Subjects

Subjects :
Medicine
Science

Details

Language :
English
ISSN :
19326203
Volume :
19
Issue :
11
Database :
Directory of Open Access Journals
Journal :
PLoS ONE
Publication Type :
Academic Journal
Accession number :
edsdoj.846b61e425d5472dbbf8a3f6d23b5e56
Document Type :
article
Full Text :
https://doi.org/10.1371/journal.pone.0310577