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Highly Specific Peptide-Mediated Cuvette-Form Localized Surface Plasmon Resonance (LSPR)-Based Fipronil Detection in Egg

Authors :
Jingon Yoo
Soobin Han
Bumjun Park
Sonam Sonwal
Munirah Alhammadi
Eunsu Kim
Sheik Aliya
Eun-Seon Lee
Tae-Joon Jeon
Mi-Hwa Oh
Yun Suk Huh
Source :
Biosensors, Vol 12, Iss 11, p 914 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Herein, we have developed peptide-coated gold nanoparticles (AuNPs) based on localized surface plasmon resonance (LSPR) sensor chips that can detect fipronil with high sensitivity and selectivity. The phage display technique has been exploited for the screening of highly specific fipronil-binding peptides for the selective detection of the molecule. LSPR sensor chips are fabricated initially by attaching uniformly synthesized AuNPs on the glass substrate, followed by the addition of screened peptides. The parameters, such as the peptide concentration of 20 µg mL−1 and the reaction time of 30 min, are further optimized to maximize the efficacy of the fabricated LSPR sensor chips. The sensing analysis is performed systematically under standard fipronil solutions and spike samples from eggs. The developed sensor has shown excellent sensitivity towards both standard solutions and spike samples with limit of detection (LOD) values of 0.01 ppb, respectively. Significantly, the developed LSPR sensor chips offer distinct features, such as a facile fabrication approach, on-site sensing, rapid analysis, cost-effectiveness, and the possibility of mass production, in which the chips can be effectively used as a promising and potential on-site detection tool for the estimation of fipronil.

Details

Language :
English
ISSN :
12110914 and 20796374
Volume :
12
Issue :
11
Database :
Directory of Open Access Journals
Journal :
Biosensors
Publication Type :
Academic Journal
Accession number :
edsdoj.84b7acc8c2c44f70822769b0ef0dae2a
Document Type :
article
Full Text :
https://doi.org/10.3390/bios12110914