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Bifidobacteria BbC50 Fermentation Products Induce Human Cd4 Regulatory T Cells with Antigen-Specific Activation and Bystander Suppression
- Source :
- European Journal of Inflammation, Vol 12 (2014)
- Publication Year :
- 2014
- Publisher :
- SAGE Publishing, 2014.
-
Abstract
- Probiotic bacteria have been shown to have health benefits in various situations (inflammation, allergy, infection). We previously showed that a bacteria-free fermentation product of Bifidobacterium breve C50 (BbC50sn) induced high IL-10 secretion by human dendritic cells. As IL-10 is a regulatory cytokine, the aim of the present study was to examine whether DCs cultured in the presence of BbC50sn could induce regulatory T cells in an allogeneic context. Purified CD4 + CD25 − human T cells were co-cultured with allogeneic BbC50sn-treated dendritic cells for 4 weeks. The T cell population (BbC50sn-T) was analysed both at phenotypical and functional [ability to inhibit a mixed lymphocyte reaction (MLR)] levels. We showed that T lymphocytes acquired phenotype characteristics of regulatory T cells after 4 weeks of co-culture with BbC50sn-DCs, and inhibited in vitro T lymphocyte proliferation and IFN-γ production in an MLR. Transwell experiments demonstrated that this suppressive activity was not T cell contact-dependent but probably mediated by a soluble factor. Although BbC50sn-T cells secreted significant amounts of IL-10 and TGF-β, their suppressive effect is most likely not mediated through these cytokines. This is, to our knowledge, the first demonstration of in vitro regulatory T cell induction by a bacteria-free fermentation product in an allogeneic context.
- Subjects :
- Medicine
Subjects
Details
- Language :
- English
- ISSN :
- 1721727X
- Volume :
- 12
- Database :
- Directory of Open Access Journals
- Journal :
- European Journal of Inflammation
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.8a74fe30c7f9441782089cda8ab21373
- Document Type :
- article
- Full Text :
- https://doi.org/10.1177/1721727X1401200116