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Comparative Study on Different Recovery Periods of the Spermatogenic Dysfunction Mouse Model Induced by Cyclophosphamide

Authors :
MA Jingwei
LI Gen
YANG Yang
ZANG Caixia
BAO Xiuqi
ZHANG Dan
Source :
Shiyan dongwu yu bijiao yixue, Vol 43, Iss 2, Pp 112-123 (2023)
Publication Year :
2023
Publisher :
Editorial Office of Laboratory Animal and Comparative Medicine, 2023.

Abstract

ObjectiveTo compare and evaluate the improvement degree of spermatogenic dysfunction mice at different recovery periods after cyclophosphamide modeling.MethodsForty-eight male ICR mice aged 4-5 weeks with the body weight of approximately 18-20 g were randomly divided into three control groups and three model groups, with 8 mice in each group. Each mouse of three model groups was intraperitoneally injected with 60 mg/kg cyclophosphamide continuously from the 1st to 7th day of the experiment, while each mouse of three control groups was intraperitoneally injected with the corresponding volume of normal saline. Then these mice were continued to be fed for another 7, 14 and 21 days after cyclophosphamide injection, respectively. A corresponding control group was set for each model group. The mice in each group were sacrificed after blood collection through orbital veins at corresponding time points. Testis, epididymis and seminal vesicle were taken and weighed, and their reproductive organ indexes were calculated. Histopathological changes of testis and epididymis were compared after HE staining.Sperm quality analysis was used to determine sperm-related indexes. Serum reproductive hormone content, testicular oxidative stress level and testicular signature enzyme activity were detected by ELISA and related kits.Results Compared with the control group, on the 7th, 14th and 21st day after cyclophosphamide treatment, the testicular index of mice in the model group decreased significantly (P

Details

Language :
Chinese
ISSN :
16745817
Volume :
43
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Shiyan dongwu yu bijiao yixue
Publication Type :
Academic Journal
Accession number :
edsdoj.8b219515d364f46adca7df9f411581e
Document Type :
article
Full Text :
https://doi.org/10.12300/j.issn.1674-5817.2022.167