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Administration of microencapsulated Enterococcus faecium ABRIINW.N7 with fructo-oligosaccharides and fenugreek on the mortality of tilapia challenged with Streptococcus agalactiae

Administration of microencapsulated Enterococcus faecium ABRIINW.N7 with fructo-oligosaccharides and fenugreek on the mortality of tilapia challenged with Streptococcus agalactiae

Authors :
Yousef Nami
Mahdi Kahieshesfandiari
Gilda Lornezhad
Amir Kiani
Daniel Elieh-Ali-Komi
Mahdieh Jafari
Mehdi Jaymand
Babak Haghshenas
Source :
Frontiers in Veterinary Science, Vol 9 (2022)
Publication Year :
2022
Publisher :
Frontiers Media S.A., 2022.

Abstract

We investigated the probiotic potential of a microencapsulated Enterococcus faecium ABRIINW.N7 for control of Streptococcus agalactiae infection in hybrid (Oreochromis niloticus × Oreochromis mossambicus) red tilapia. A two-phase experiment approach was completed in which E. faecium bacteria were propagated, from which a culture was isolated, identified using molecular techniques, and microencapsulated to produce a stable commercial fructooligosaccharide (FOS) and fenugreek (Fk) product of optimal concentration. The FOS and Fk products were assessed in a 90-days in vivo challenge study, in which red hybrid tilapia were allocated to one of five treatments: (1) No Streptococcus agalactiae (Sa) challenge (CON); (2) Sa challenge only (CON+); (3) Sa challenge in a free cell (Free Cell); (4) Sa challenge with 0.8% (w/v) Alginate; (5) Microencapsulated FOS and Fk. In vitro results showed high encapsulation efficiency (≥98.6 ± 0.7%) and acceptable viability of probiotic bacteria within the simulated fish digestive system and high stability of viable cells in all gel formulations (34 < SR%

Details

Language :
English
ISSN :
22971769 and 17394120
Volume :
9
Database :
Directory of Open Access Journals
Journal :
Frontiers in Veterinary Science
Publication Type :
Academic Journal
Accession number :
edsdoj.8beeb60c17394120a5ae95cd680987dc
Document Type :
article
Full Text :
https://doi.org/10.3389/fvets.2022.938380