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Phototactic Changes in Phthorimaea absoluta Long-Wavelength Opsin Gene Mutants (LW2−/−) and Short-Wavelength Opsin Gene Mutant (BL−/−) Strains

Authors :
Yanhong Tang
Xiaodi Wang
Jianyang Guo
Nianwan Yang
Dongfang Ma
Fanghao Wan
Chi Zhang
Zhichuang Lü
Jianying Guo
Wanxue Liu
Source :
Insects, Vol 15, Iss 6, p 433 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Phthorimaea absoluta (Meyrick) is an invasive pest that has caused damage to tomatoes and other crops in China since 2017. Pest control is mainly based on chemical methods that pose significant threats to food safety and environmental and ecological security. Light-induced control, a green prevention and control technology, has gained attention recently. However, current light-trapping technology is non-specific, attracting targeted pests alongside natural enemies and non-target organisms. In this study, we characterized the phototactic behavior of tomato leaf miners for the development a specific light-trapping technology for pest control. In situ hybridization revealed opsin expression throughout the body. Furthermore, we investigated the tropism of pests (wild T. absoluta, Toxoptera graminum, and Bemisia tabaci) and natural enemies (Nesidiocoris tenuis and Trichogramma pintoi) using a wavelength-lamp tropism experiment. We found that 365 ± 5 nm light could accurately trap wild P. absoluta without trapping natural enemies and other insects. Finally, we analyzed the phototactic behavior of the mutant strains LW2(−/−) and BL(−/−). LW2 and BL mutants showed significant differences in phototactic behavior. The LW2(−/−) strain was attracted to light at 390 ± 5 nm and the BL(−/−) strain was unresponsive to any light. Our findings will help to develop specific light-trapping technology for controlling tomato leaf miners, providing a basis for understanding pest population dynamics and protecting crops against natural enemies.

Details

Language :
English
ISSN :
20754450
Volume :
15
Issue :
6
Database :
Directory of Open Access Journals
Journal :
Insects
Publication Type :
Academic Journal
Accession number :
edsdoj.8bf991e985144fb69dcd646cb42a092f
Document Type :
article
Full Text :
https://doi.org/10.3390/insects15060433