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A Microfluidic Paper-Based Lateral Flow Device for Quantitative ELISA

Authors :
Ashutosh Kumar
Cameron Hahn
Stephen Herchen
Alex Soucy
Ethan Carpio
Sophia Harper
Nassim Rahmani
Constantine Anagnostopoulos
Mohammad Faghri
Source :
Micro, Vol 4, Iss 2, Pp 348-367 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

This study presents an innovative lateral flow microfluidic paper-based analytical device (μPAD) designed for conducting quantitative paper-based enzyme-linked immunosorbent assays (p-ELISA), seamlessly executing conventional ELISA steps in a paper-based format. The p-ELISA device utilizes a passive fluidic circuit with functional elements such as a multi-bi-material cantilever (B-MaC) assembly, delay channels, and a buffer zone, all enclosed within housing for autonomous, sequential loading of critical reagents onto the detection zone. This novel approach not only demonstrates a rapid assay completion time of under 30 min, but also boasts reduced reagent requirements, minimal equipment needs, and broad applicability across clinical diagnostics and environmental surveillance. Through detailed descriptions of the design, materials, and fabrication methods for the multi-directional flow assay (MDFA), this manuscript highlights the device’s potential for complex biochemical analyses in a user-friendly and versatile format. Analytical performance evaluation, including a limit of detection (LOD) of 8.4 pM for Rabbit IgG, benchmarks the device’s efficacy compared to existing p-ELISA methodologies. This pioneering work lays the groundwork for future advancements in autonomous diagnostics, aiming to enhance global health outcomes through accessible and reliable testing solutions.

Details

Language :
English
ISSN :
26738023
Volume :
4
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Micro
Publication Type :
Academic Journal
Accession number :
edsdoj.8bfbca81ef944494aa1f8264593fb592
Document Type :
article
Full Text :
https://doi.org/10.3390/micro4020022