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Functional analysis of the interferon-stimulated response element of porcine circovirus type 2 and its role during viral replication in vitro and in vivo

Authors :
Gu Jinyan
Zhang Yu
Lian Xue
Sun Hailiang
Wang Jingman
Liu Weiting
Meng Gang
Li Peng
Zhu Dan
Jin Yuexin
Cao Ruibing
Source :
Virology Journal, Vol 9, Iss 1, p 152 (2012)
Publication Year :
2012
Publisher :
BMC, 2012.

Abstract

Abstract Background Porcine circovirus type 2 (PCV2) is associated with post-weaning multi-systemic wasting syndrome (PMWS) in young weaned pigs. Immune stimulation was found to activate the replication of PCV2 and exacerbate the clinical outcome of the infection. Proper amount of interferon-α (IFN-α) is able to enhance PCV2 infection and production in Porcine kidney-15 (PK-15) cells when administered after inoculation. Methods In the present study, luciferase reporter assays, construction of mutant viruses, Analysis the replication efficiency and the response to IFN-α treatment in PK-15 cells and animal experiments were carried out to analyze the function of interferon-stimulated response element (ISRE) of PCV2 and its role during viral replication in vitro and in vivo. Results A functional viral ISRE sequence, 5′-CTGAAAACGAAAGA-3′, was identified in Rep gene promoter (Prep) of PCV2. PCV2 Prep is composed of two mini promoters, the proximal one span the sequence +1 to -106, containing an ISRE while the distal mini promoter is composed of three tandem GC box like sites locate at -85 to -194. It was demonstrated that viral ISRE is necessary for porcine IFN-α initiated luciferase expression enhancement and it plays an important role in affecting the replication efficiency of PCV2 in vivo and in vitro. Conclusions These findings provide a theoretical basis for the Phenomenon of immunostimulation is able to enhance PCV2 infection, and improve the understanding of the complicated mechanisms involved in the host and pathogen interactions of PCV2.

Details

Language :
English
ISSN :
1743422X
Volume :
9
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Virology Journal
Publication Type :
Academic Journal
Accession number :
edsdoj.8cd4d3472feb4bbda09185b8fa4b116e
Document Type :
article
Full Text :
https://doi.org/10.1186/1743-422X-9-152